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5_Biol221 Lecture 5 01_27_11

5_Biol221 Lecture 5 01_27_11 - Suppose that several that...

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1 Suppose’that’several’that’you’have’induced’ several’rounds’of’meiosis.’Using’PCR’primers’that’ flank’the’CAG’repeats,’you’get’the’following’ results’in’tes±ng’the’Frst’polar’body.’ 1 st round of ovula.on day 0 :’2’products’both’with’24’CAG’repeats.’ 2 nd round of ovula.on day 0: 2’products’both’that’have’44’CAG’repeats’ 3 rd round of ovula.on day 0: 2’products’1’with’24’and’the’other’with’44’CAG’repeats’ Explain’the’signiFcance’of’these’results.’Which’rounds’produced’an’egg’without’ the’disease’causing’allele?’How’would’you’determine’whether’to’use’the’egg’produced’ in’round’3? The’next’slide’contains’a’diagram’of’meiosis’for’your’convenience.’
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4 44/44’ Mother’is’a’44/24’heterozygote’ 24/24’ 24/24’ 44/44’ 24/44’ 24/44’ 1)’ 2)’ 3)’ What’is’the’genotype’of’the’egg?’
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5 Mother’is’a’44/24’heterozygote’ 24/44’ 44’ 44’ 44’ 44’ 24’ 24’ 24’ 24’
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6 Mother’is’a’44/24’heterozygote’ 24/44’ 44’ 44’ 44’ 44’ 24’ 24’ 24’ 24’ 24?’or’44?’
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FronAers’in’Bioscience’14,’1471-1478,’January’1,’2009’ GeneAc’cell’culture’screens’reveal’ mitochondrial’apoptosis’control’
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Box’2’|’The’basis’of’seed’mutagenesis’in’Arabidopsis’ From’the’following’ar±cle:’ The’art’and’design’of’gene±c’screens:’Arabidopsis’thaliana’ Damian’R.’Page’&’ Ueli’ Grossniklaus’ Nature’Reviews’Gene±cs’3,’124-136’(February’2002)’ doi:10.1038/nrg730’
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9 Forward’GeneAcs’in’Model’Systems’
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What’does’dominance’tell’you’about’the’ funcAon’of’the’wild-type’allele?’ Suppose’you’are’studying’a’gene’involved’in’trichome’ formaAon’in’ Arabidopsis ’and’you’have’found’two’separate’ unlinked’recessive’mutaAons’that’have’too’many’branches.’
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11 MUT1’ MUT2’ Trichome’branching’ Since’both’alleles’are’recessive’ we’can’say’that:’
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12 Genes’involved’in’trichome’formaAon’
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13 MUT1’ MUT2’ EndoreduplicaAon’ Since’both’alleles’are’recessive’ we’can’say’that:’
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14 SomaAc’Reprogramming’of’Ovaries’to’Testes’ by’recessive’mutaAons’in’Foxl2’ ’These’microscopy’images’show’the’cellular’reprogramming’uncovered’by’EMBL’scienAsts.’On’the’ leC’is’an’ovary’of’a’normal’adult’female’mouse,’with’a’close-up’(top’leC)’showing’the’typical’ female’granulosa’cells.’When’the’Foxl2’gene’was’silenced’in’these’cells’(right,’top’right:’close-up),’ they’took’on’the’characterisAcs’of’Sertoli’cells,’the’cells’normally’found’in’testes’of’male’mice.’ Mathias’Treier’
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15 SomaAc’Reprogramming’of’Ovaries’to’Testes’ by’recessive’mutaAons’in’Foxl2’ Mathias’Treier’ FOSL’ Male’Germ-line’Character’
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16
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17 Ectopic’expression’of’Antennapedia’ Neomorphic’Muta±ons’in’ Antennapedia’
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5_Biol221 Lecture 5 01_27_11 - Suppose that several that...

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