23_Biol221_04_14_2-11

Fosb mice test three to further rule out cognilve

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Unformatted text preview: learned over Lme. fosb mice TEST TWO: Each mother was deprived of her pups for one hour and then three pups were returned and placed in three corners of the cage. In more tests, it was also shown that the nurturing defect was not sex specific. The effect held for both male and female mutant mice. The fact that it was not sex specific suggests that the defect reflects a funcLon of FosB in the brain circuitry in response to the presence of pups. fosb mice TEST THREE: To further rule out cogniLve defects, the mice were subjected to the hidden plalorm test in the Morris water maze. The mutants performed the same as wild ­type mice. Mutant mice did not appear to suffer from any drasLc cogniLve or intellectual defects in relaLon to the performance of the wild ­type mice. fosb mice TEST FOUR: To eliminate the possibility of sensory defect, the olfactory senses were tested. Mutant mice were essenLally indisLnguishable in their ability to detect isovaleric acid and pentadecalactone. The hypothalmus was also ruled out as a source of the defect because all other processes regulated by the hypothalmus (adaptaLon to cold, male aggression, locomotor acLvity, eaLng, sexual behavior, etc.) were normal. This suggests that the FosB controls a region of the brain whose funcLon is to control the nurturing response. fosb mice TEST FIVE: Does the presence of the pups illicit Fosb expression? Yes The final test tries to explain the expression of FosB in the brain when the ability to nuture is controlled. The fact that there is a specific nuturing defect in the FosB mutant mice relates that FosB is controlled in the brain within a nuturing response region. To test the possibility that the newborn pups trigger the inducLon of FosB in the brain of the mother, the affect of pup presentaLon was examined. SecLons of the brain were stained with AnL ­FosB anLbodies before, two, and six hours ader the pups were presented to the mutant mothers. The results demonstrated that FosB was clearly visible in the preopLc area of the hypothalmus (POA) ader a six hour exposure to the pups. From previous studies, showing that POA is criLcal for nurturing behavior, and that the disrupLon of the FosB leads to a nuturing defect, the studies suggest that the FosB may act with the POA neuron in the illustraLon of nuturing behavior. The analysis of the tests also showed posiLve results of FosB within several other brain regions including the main and accessory olfactory bulbs and pyriform cortex. Upon presentaLon of the pups, there was no further indicaLon of FosB in the acc...
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This note was uploaded on 01/20/2014 for the course BIOL 221 taught by Professor Gallagher,kimberly during the Spring '13 term at UPenn.

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