Lecture3

Lecture3 - CHE/BME 489 Biochemical Engineering Lecture 3...

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CHE/BME’489:’Biochemical’ Engineering’ Lecture’3’ Aerobic’Bioreactors’ 1/30/2013’
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Announcements Homework’#1’is’due’today’ Reading’assignment’#1’is’out’today’ (blackboard);’two’essays’are’due’next’ Wednesday’ Team’topic’should’be’chosen’by’today’(email’ to’TA’and’me)’ Lecture’notes’are’posted’on’blackboard’
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Last’Time How’to’read’a’scienNfc’paper’ Microbial’FermentaNon’ ±ermentor’ Prokaryotes/Eukaryotes’ ComposiNon’Formula’ Cell’growth’media’ Cell’growth’phases’ Cell’growth’expression’ ClassifcaNon’oF’ FermentaNon’ Stoichiometry’and’yield’ coefficient’ Model’the’cell’growth’ Math’ KineNcs’oF’cell’grwoth’ Ethanol’Fermentaion’ Luedeking-Piret’model’ ConNnuous’sNrred-tank’ bioreactor’
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Today s’Class CSTB’ Experiment’for’fermentaNon’of’xylose’to’2,3- butanediol’ Why’?’ Stoichiometry’ Experimental’methods’ Experimental’results’ Model’development’ QuanNFcaNon’of’oxygen’transfer’
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KineNcs’of’Cell’Growth’(notes)
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Plot’of’Monod’EquaNon
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SimulaNon’of’cell’growth’by’RK4’(see’notes)
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KineNc’constants’for’ethanol’fermentaNon
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DefniNon’oF’Ks
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Maintenance’coefficient
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SimulaNon’of’a’bath’ethanol’fermentaNon
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Constants’for’cell’growth’ Constants’for’ethanol’producNon’ Correspond to µ max Correspond to v max
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Ethanol’inhibiNon’in’the’fermentaNon
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Process’flow’diagram’for’ethanol’fermentaNon
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SimulaNon’improvement
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Luedeking-Piret’Model Another’unstructured’model’
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ConNnuous’sNrred-tank’bioreactor’(CSTB)
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Study’of’CTSB To’study’biomass’growth’rate’WRT’changes’in’ substrate’concentraNon’ To’study’biomass’growth’rate’in’response’to’ environmental’parameter’such’as’pH’ ’Substrate-limited’growth’with’a’constant’flow To’study’metabolic’flux’for’systems’biology’ models’of’cellular’metabolism’ To’deign’bioreactor’ To’operate’bioreactor’for’maximal’efficiency’
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ConNnuous’sNrred-tank’bioreactor
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Balance’for’the’growth’limiNng’substrate
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Model’of’CSTB
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Model’of’CSTB
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Model’of’CSTB
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Washout’and’maximum’biomass’output
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Aerobic’Bioreactors
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Lecture3 - CHE/BME 489 Biochemical Engineering Lecture 3...

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