Bio 4 - Unit 4: Enzymes Instructor: Cheryl Dunham Office:...

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Unit 4:  Enzymes Instructor: Cheryl Dunham Office: 102 LeFevre Hall Box: 114 LeFevre Hall FIRST EXAMINATION Thursday, February 28 th 6:30 pm Waters Auditorium
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KNOW YOUR  VOCABULARY
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Enzymes Enzymes Proteins Biological  catalysts  (substances that promote chemical  reactions) Causes  substrate  (reactants) to react and yield  product   molecules Reduce  the  activation energy  required for a reaction to occur Enzymes are  NOT   consumed  in reactions, they merely promote  them Generally have the (http://www.jonbarron.org/images/enzymes.jpg)
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Lock and Key Model Enzymes are  specific Each enzyme has a different structure These  specific structures are important to their function Each enzymes corresponds to a specific substrate that  “locks” into the  active site  of the enzyme When the substrate locks into the active site a “ substrate  + enzyme complex ” is formed and the catalytic effect  takes place If the  enzyme shape is altered , the substrate cannot fit  into the active site and the  reaction will  NOT  occur http://waynesword.palomar.edu/images/enzyme5.gif
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Lock and Key Model Enzymes are  specific When liver extract came in contact with hydrogen peroxide  (H 2 O 2  ), bubbles (a gas) were produced Catalase is a protein that converts H 2 O 2  to water and oxygen  gas Substrate? Enzyme? Products? http://waynesword.palomar.edu/images/enzyme5.gif
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Lock and Key Model Enzymes are  specific When liver extract came in contact with hydrogen peroxide  (H 2 O 2  ), bubbles (a gas) were produced Catalase is a protein that converts H 2 O 2  to water and oxygen  gas Substrate: hydrogen peroxide Enzyme: catalase Products: water and oxygen gas http://waynesword.palomar.edu/images/enzyme5.gif
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Temperature As an enzyme is heated, the rate of reaction  increases to an  optimal  level This is at the  peak  of the reaction curve If heated too much, the enzyme will  denature   (essentially melt), changing its 3-D structure Reactions  CANNOT occur in a denatured state , because the substrate will no longer fit in the active site
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pH is a measure of the number of hydrogen  ionsin a solution Since hydrogen ions are merely protons (positively  charged particles), pH is a  measure of the concentration  of charged particles  in solution Enzymes are  charged particles  too They have areas of positive and negative charge in their  surface The  attraction or loss of hydrogen ions could change the  shape of an enzyme or block the active site Remember opposite charges attract Enzymes have an  optimum  pH, corresponding to the 
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This note was uploaded on 04/08/2008 for the course BIO 1020 taught by Professor Dunham during the Spring '08 term at Missouri (Mizzou).

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Bio 4 - Unit 4: Enzymes Instructor: Cheryl Dunham Office:...

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