Practice Problems for Exam 2

17during cloning a protein is often constitutively

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Unformatted text preview: of the incoming NTP? 13)During solid phase synthesis of DNA, what is the purpose of the oxidation step? 14)During peptide synthesis, by what mechanism might racemization occur? 15)What is the purpose of molecular cloning? 16)What are plasmids and what role do they play in molecular cloning? 17)During cloning, a protein is often constitutively expressed. What does this mean? 18) What is the purpose of the solid- phase in chromatographic separations? 19)What is a HIS- tag and how does it work...
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