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Unformatted text preview: 2/2/12 Both of the genes shown below have large enhancer regions to which
the transcription factor Pax-6 can bind.
Crystallin gene Somatostatin gene What is the best explanation for why crystallin protein is found only in the
eye, while somatostatin protein is found only in the pancreas?
a. Pax6 binding is only required for transcription of one, not both, of these
b. Pax6 can act as either an enhancer or silencer.
c. Different additional transcription factors are required in each cell type.
d. The enhancer regions to which Pax6 binds are different in cells of the
pancreas cells vs. cells of the eye. The components of the signaling pathway that determine
which side of a Drosophila embryo will be dorsal were
identified by application of (Choose the most useful
a) biochemical analysis of gene product activities.
b) epistasis testing.
c) forward genetics.
d) reverse genetics.
e) mosaic analysis. Understanding embryonic development
Development requires establishment of
first in the early embryo, later in other cells
"Give me one asymmetry, and I can explain development."
Sydney Brenner, 1960s Using genetics to understand development
Forward genetics with certain organisms is a
powerful approach to finding the genes that
control developmental processes.
e.g. screening for mutants, mapping, gene identification,
protein identification Reverse genetics is a powerful approach to
defining functions of cloned genes, in addition
to experiments using...
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This note was uploaded on 01/22/2014 for the course MCB 305 taught by Professor Schroeder during the Fall '11 term at University of Arizona- Tucson.
- Fall '11