Eg targeted knockouts rnai reporter constructs mosaic

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Unformatted text preview: mutants. e.g. Targeted knockouts, RNAi, reporter constructs, mosaic and chimera experiments. The order of interactions among gene products involved in vulval fate determination was determined by -- ? (Choose the most useful approach): a) biochemical analysis of gene product activities. b) epistasis testing. c) forward genetics. d) reverse genetics. e) mosaic analysis. Cells in an embryo become "determined" for different developmental fates by two major mechanisms: asymmetric segregation of cytoplasmic factors during cell division, and in response to signals from other cells. But now that we understand the mechanisms, both are just gene expression and signaling pathways all over again. 2 12/2/12 Maternal-effect lethal mutants P0 +/+ F1 m/+ Mesoderm arises near the equator (in the marginal zone) of the Xenopus blastula because mutagenize “m” represents a loss of function in a maternal gene required for survival Mate F1 animals together (or allow to self fertilize) F2 embryos: +/+ m/+ embryo will live live m/m ? Question: If m is a strict maternal effect lethal mutation, m/m embryo will a) live. a) animal hemisphere cells in this region are autonomously determined to become mesodermal precursors. b) vegetal hemisphere cells in this region are autonomously determined to become mesodermal precursors. c) animal hemisphere cells in this region receive signals from underlying vegetal hemisphere cells and become mesodermal precu...
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This note was uploaded on 01/22/2014 for the course MCB 305 taught by Professor Schroeder during the Fall '11 term at University of Arizona- Tucson.

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