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Unformatted text preview: n.net/ebooks/wsug encheml1/lab_3/manual.html 3/7 4/15/13 Lab 3 - Solutions and Spectr oscopy CuSO4 · 5 H2O is listed as toxic and an irritant. Toxic substances are hazardous to health when breathed,
swallowed or in contact with the skin. An irritant may have a temporary irritating effect on skin, eyes, respiratory
tract, etc. If you come in contact with the solid, you should gently brush off the affected area with a paper towel
and then flush the area with water. If you come in contact with a solution of copper(II) sulfate, you should flush
the affected area with water. Waste Disposal
Solutions containing copper ions must be placed in the waste bottle in the lab. Designate a "waste copper"
beaker and set it aside for use during your lab. You can put the small samples of copper solution you will make
in this and empty it into the waste bottle at the end of class, instead of going back and forth to the waste bottle.
Always remember not to overfill the waste bottle. If your waste bottle is full, please alert your lab instructor. Prior to Class
Please read the following section of Lab Safety and Practices:
Good Lab Practices
Please read the following section in Lab Equipment:
Please review the following videos:
Using an Analytical Balance
Using a Volumetric Flask
Laboratory Safety Lab Procedure
Please print the worksheet for this lab. You will need this sheet to record your data.
www.webassig n.net/ebooks/wsug encheml1/lab_3/manual.html 4/7 4/15/13 Lab 3 - Solutions and Spectr oscopy Part A: Determination of the Concentration of a Copper(II) Ion Solution
A spectrophotometer will be set up in your work area. Make sure it is turned on and allow it to warm up.
While the spectrophotometer is warming up, obtain two serological pipets, and set up a beaker for waste Cu2+
Obtain about 15 mL stock Cu2+ solution. Write its exact concentration in Data Table A.
Once the spectrophotometer is warmed up, take a blank spectrum of deionized water in a vial as described in
the MicroLab spectrophotometer instructions provided in lab.
Condition the pipet you will be using for the Cu2+ solution as shown in the pipet video under instrumentation and
as described in the Volumetric Glassware section of the Introductory Material of this lab manual. Dispense the
required volumes of Cu2+ solution (1.2 mL) and deionized water (4.8 mL) for...
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- Spring '14