MIMM324_Phage_I

Envelope whiskers lifecycle lygc burst cells or

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Unformatted text preview: •  Bacteriophage come in many shapes and sizes Bacteriophage Classification •  Nucleic Acid Content •  dsDNA, ssDNA, ssRNA, dsRNA •  Virion Shape and Size •  Head  ­ Icosahedral, Prolate •  Tail  ­ ContracGle, Non ContracGle •  Filamentous •  Envelope •  Whiskers •  Lifecycle •  LyGc (Burst Cells) or Non ­LyGc or Temperate (Integrates into host) Bacteriophage come in all shapes T4 λ T7 All Images from ICTVdB Bacteriophage come in all shapes SSV -1 MS2 φ6 F1 All Images from ICTVdB φX174 Bacteriophage come in a range of sizes MS2 Lambda φX174 T7 All Images created using Pymol or Chimera All data from EMDB and PDB 10nm T4 Bacteriophage come in a range of sizes Filamentous phage f1 (860nm*6nm) All Images created using Pymol or Chimera All data from EMDB and PDB = 100nm What has been discovered using Bacteriophage? •  Plaque assay ( D'Herelle 1917) •  Nature of a virus life cycle (Ellis & Delbrück 1939) •  Nature and types of geneGc mutaGons (Luria & Delbrück fluctuaGon test 1943) •  ConfirmaGon that gene...
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