{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Ci binds or3 inhibigng ci expression 35 10 or3 ci

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: nto single genome lengths during head packaging 1 T7 genome 150bp terminal repeats Concatermer λ (Lambda) •  Siphoviridae •  dsDNA •  40 ­60kbp •  ssDNA extenGon (Cohesive ends) •  Temperate bacteriophage •  Lysogenic phase •  Icosahedral Head •  63nm diameter •  Tube like tail •  135nm long •  non ­contracGle •  Receptor – sugar transport proteins LamB (maltose trasporter) Ross Inman, University of Wisconsin, Madison Lysis vs Lysogeny v Lysis s Lysogeny Induction of prophage Controlling λ lytic transcription program cIII N cI cro cII O P Q tL1 PL PR NUTR NUTL +N  ­N tR1  ­N +N N = anBterminator Q S R Rz Nu1 A PR1 tR1  ­Q +Q Long Transcript encoding LyBc genes Establishing Lysogeny RNA polymerase binds acBvaBng cI expression establishing lysogeny cI cI mRNA PR OR3  ­10  ­35 PRM CI repressor OR2  ­35 OR1 ­ 10 cro mRNA cro cI Activation inhibit PR and PL Expression cI binds PL inhibiGng transcripGon OL2  ­35 OL3 cI binds OR3 inhibiGng cI expression  ­35  ­10 OR3 cI Repress...
View Full Document

{[ snackBarMessage ]}