Influenza virus III

Influenza virus III

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Unformatted text preview: some RBC No quan6fica6on of the presence of virus HemmaggluEnaEon assay (HA) NO Virus rescue Virus rescue Virus diluEons 1/2 1/4 1/8 1/16 1/32 1/64 1/128 1/256 No virus rescue Virus rescue Plaque assay Advantages Reliable Quan6ta6ve detec6on of influenza virus Disadvantages Time consuming Require special media Difficult to perform as compared with other assays Plaque assay 0.1 ml 0.1 ml 0.1 ml 0.1 ml 0.1 ml 0.1 ml 0.1 ml Virus stock 0.1 ml 0.9 ml 0.9 ml 0.9 ml 0.9 ml 0.9 ml 0.9 ml 0.9 ml Virus diluEon - 1 - 2 - 2 - 3 - 4 - 5 - 6 - 7 - 3 - 4 - 5 - 6 - 2 - 5 - 7 - 3 - 6 - 4 - 7 Immunofluorescence Advantages Relailable Qualita6ve detec6on of influenza virus Very sensi6ve Disadvantages Requires the access to an6bodies for viral detec6on Time consuming Require the use of appropriate nega6ve and posi6ve controls Require access to a fluorescent microscope Difficult to perform as compared with other assays Immunofluorescence No virus Presence of virus Experimental tools and methods 1) Methods for detecEon of rescue virus: - Cytopathic effect (cpe) - HemaggluEnaEo...
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This document was uploaded on 01/26/2014.

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