Influenza virus III

Mice ferrets guinea pigs convenient human viruses

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Unformatted text preview: uenza virus will prevent binding to RBC and hemaggluEnaEon is inhibited A) B) A) HI B) NO HI Experimental tools and methods 1) Methods for detecEon of rescue virus: - Cytopathic effect (cpe) - HemaggluEnaEon (HA) assay - Plaque assay - Immunofluorescence (IF) assay 2) Influenza virus growth in eggs 3) HemaggluEnaEon inhibiEon (HI) assay 4) Influenza virus minigenome assay 5) Influenza virus animal models Influenza virus minigenome assays •  Common technique to study replicaEon and transcripEon Protein expression plasmids NP PA PB1 PB2 vRNA expression plasmids CAT GFP LUC Reporter gene expression Influenza virus minigenome assay A) B) CAT GFP C) Fold Increase Negative control D) Other reporters 70 LUC Positive control 60 50 Red fluorescent protein (RFP) Alkaline phosphatase Gaussia luciferase 40 30 20 10 0 Negative Positive control control Experimental tools and methods 1) Methods for detecEon of rescue virus: - Cytopathic effect (cp...
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