Influenza virus III

Cavity air sac embryo make a hole in the labeled line

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Unformatted text preview: k for dead eggs before and after viral infection. Before infection a dead egg can be easily spotted by the absence of blood vessels as well as the absence of embryo mobility. When candled, live embryos move. After viral infection a dead egg (probably related to influenza virus infection) will be easily spotted by the bad appearance of the egg as seen by the smaller and bloody volume of allantoic fluid. Avian viruses do not growth well in eggs. InfecEon of chicken embryonated eggs PreparaEon of eggs shell allantoic cavity air sac embryo Virus inoculaEon IncubaEon yolk albumen amnioEc cavity Harvest of allantoic fluid InfecEon of chicken embryonated eggs PreparaEon of eggs shell allantoic cavity air sac embryo - Candle the egg to check the eggs - Delimitate the allantoic cavity yolk -   Mark the line between the air sac and the allantoic cavity albumen amnioEc cavity InfecEon of chicken embryonated eggs Virus inoculaEon shell allantoic cavity air sac embryo - Make a hole in the labeled line - Inoculate the virus in the allantoic cavity yolk -   Close the hole with wax to keep the eggs under sterile condi6ons. albumen amnioEc cavity InfecEon of chicken e...
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