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Influenza virus III

Culture supernatants andor the allantoic uid of

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Unformatted text preview: of cpe by observed May drive to wrong conclusions No quan6fica6on of the presence of virus Cytopathic effect (cpe) No cpe cpe “satellite formaEon” HemaggluEnaEon (HA) assay Hemagglutination assay (HA) is routinely used to detect the presence of rescued virus in MDCK tissue culture supernatants and/or the allantoic fluid of harvested eggs. The presence of virus induces hemagglutination of RBC while the absence of virus allows the formation of a red pellet in the bottom of the well. In the case of influenza virus, it is believed that approximately 103-104 plaque forming units (pfu) are required to give a positive signal in the HA assay. HemaggluEnaEon (HA) assay Advantages The most common method for detec6on of influenza virus Reliable Qualita6ve detec6on of influenza virus Disadvantages Requires the access to RBC Some influenza virus do not hemagglu6nate well...
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