2006 looked at the location of nuclear localization

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Unformatted text preview: ks) The distribution of phosphatidylserine is asymmetric (1 pt), occurring generally on the inner (cytosolic) leaflet of the membrane and only in occasional spots on the outer surface of the plasma membrane. [This differential distribution of phosphatidylserine could be due to localized phosphatidylserine flippase activity.; (need to state something about barrier, switching or flipping1 pt) Student answer: Asymmetric distribution of phospholipid is caused by flippases. Page 4 of 6 Phospholipids cannot flip to the other side of the leaflet by itself, new phospholipids on the inside of the leaflet must be flipped by flippases Therefore flippases don’t flip phosphatidyl serine very much to the outside leaflet. Question 5 (6 marks) Wodrich et al. (2006) looked at the location of nuclear localization signals (NLS) within Adenovirus protein pVII. This is one of their experiments: • Full length and deletion mutants of pVII were fused to GFP (green fluorescent protein) and injected into the cytoplasm of cells. • Injected cells were viewed using fluorescence microscopy. 1: Full length pVII (amino acids 1 to 198) 2: Amino acids 1 to 81 (of primary sequence) 3: Amino acids 82 to 198 (of primary sequence) a) What do the data show? (3 marks) 1. full length pVII is detected...
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This note was uploaded on 01/27/2014 for the course BIOL 200 taught by Professor Unknown during the Winter '97 term at UBC.

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