Midterm2011_AnswerKeycorrected

Page 5 of 6 question 6 15 marks

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: in the nucleus but not the cytoplasm (or construct #1 detected in nucleus but not the cytoplasm) 2. pVII with only aa 1 ­81 is found only in the cytoplasm (or construct #2 detected only in the cytosol) 3. pVII with only aa 82 ­198 is found only in the nucleus (or construct #3 detected only in the nucleus) b) What can you conclude from this experiment? Explain (3 marks) The NLS in pVII is contained within aa 82 198, but not within aa 1 81 (1 pt),. This is because: full length pVII is detected in the nucleus but not the cytoplasm, indicating that pVII is imported into the nucleus; aa 1 ­81 is found only in the cytoplasm, indicating that there is no signal for nuclear import in this region of pVII; aa 82 ­198 is found only in the nucleus, indicating that this region of pVII contains a signal for nuclear import (2 pts),. Page 5 of 6 Question 6. (15 marks) ESSAY. (answer in exam booklet) Binding of proteins to DNA and RNA is the most essential process for nuclear organization and function. Discuss with at least 2 relevant examples. 5 marks – Essay structure and logic: Thesis statement on common themes (2), grammar, spelling and overall clarity (2), organ...
View Full Document

This note was uploaded on 01/27/2014 for the course BIOL 200 taught by Professor Unknown during the Winter '97 term at The University of British Columbia.

Ask a homework question - tutors are online