Midterm2011_AnswerKeycorrected

Presence of annexin v is detected by bright

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Unformatted text preview: er (equivalent in orientation of the intact plasma membrane); and (2) inside ­out membrane vesicles in which the extracellular leaflet faces the interior of the vesicles and the cytoplasmic leaflet faces the buffer. Annexin V is a protein that binds to phosphatidylserine, one of the phospholipids present in cell membranes. Fluorescently labeled annexin V is added to the buffer containing each of the vesicle preparations (ROVs and IOVs), and incubated briefly to allow labelled annexin V binding to the target. The vesicles are then washed to remove unbound annexin V and then examined by fluorescence microscopy. Presence of annexin V is detected by bright fluorescence against a dark background, as shown above. a) What do the data show? (2 marks) Fluorescence staining is much more intense for the IOVs compared to the ROVs (1 pt). Or: The data show uniform green fluorescence on IOVs and small discrete spots of gr...
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This note was uploaded on 01/27/2014 for the course BIOL 200 taught by Professor Unknown during the Winter '97 term at UBC.

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