Also accepted if bands unlabeled but banding pattern

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: rotein bands on the gel image below. Lane 1: intact cell with no protease treatment Lane 2: hole in cell (broken cell) with no protease treatment Lane 3: membrane solubilized completely by detergent first followed by protease treatment Lane 4: intact cell + treatment with a solution of high salt concentration + no protease treatment Lane 5: intact cells + protease treatment Lane 6: hole in the cell (broken cell) + protease treatment One mark for each lane – all or none; Lane 6 bands B and C shown shifted down partly also accepted. If bands unlabeled, but banding pattern matches answer key, then marks out of 3 only Page 3 of 6 Question 4 (6 marks) Two membrane preparations have been derived from the plasma membrane of red blood cells and are present in a buffer solution: (1) right ­side ­out membrane vesicles, in which the extracellular leaflet of the vesicle faces the buff...
View Full Document

This note was uploaded on 01/27/2014 for the course BIOL 200 taught by Professor Unknown during the Winter '97 term at The University of British Columbia.

Ask a homework question - tutors are online