200 lecture 2

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Unformatted text preview: belprize.org/nobel_prizes/chemistry/ 3. GFP ­fusion allows proteins to be tracked throughout the living cell by confocal microscopy Fig. 15 ­31 Videos 15.7 & 15.8 Q16. Confocal immunofluorescence micrograph. What is the problem with this image? A. Staining B. 3-D image C. Resolution Electron microscopy for higher resolu:on Resolu:on limit = 0.2 nm Images are black and white TEM of Plant microtubules (diameter = 25 nm) SEM of Muskox tooth surface Can you draw the path of light through the microscope? SimilariNes & differences between LM, TEM & SEM LM You will not be tested on this! TEM SEM Transmission electron microscopy (TEM) Advantages  ­Details of cytoplasm can be seen (samples are cut into thin sec:ons) Arabidopsis thaliana seed coat cell Disadvantages:  ­Cells must be dead  ­Complex specimen prepara:on  ­ difficult to know 3D shape of structures. Remember the 3rd dimension Scanning electron microscopy (SEM) :  ­Can view surfaces (images appear 3D) Disadvantages:  ­Cells must be dead Muscle :ssue from a cow...
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This note was uploaded on 01/27/2014 for the course BIOL 200 taught by Professor Unknown during the Winter '97 term at The University of British Columbia.

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