200 lecture 2

Genecsutaheducontentbegincellsscale 100 m which

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Unformatted text preview: D model of a cellular structure. Scale of things: shi< your mind from meters to microns (= micrometers) Animal cells ­ rat fibroblasts 10 µm Plant cells ­leaf Cool Flash anima:on: h\p://learn.gene:cs.utah.edu/content/begin/cells/scale/ 100 µm Which are closest in size? 1.  2.  3.  4.  5.  6.  Ribosome (diameter) Nucleus (diameter) Membrane thickness Microtubule (diameter) Mitochondrion (diameter) DNA molecule (diameter) A.  2 and 5 B.  1 and 2 C.  3 and 4 D.  1 and 4 E.  3 and 6 Size tutorial: Resources→Study Aids→ Background→Sizes Types of Microscopy Panel 1 ­1 (both edi:ons) Electron Microscopy [EM] Light Microscopy [LM] Transmission EM Brigheield LM Specialized LM epifluorescence Scanning EM Confocal What can we see? Fig 1 ­6 (both edi:ons) Magnifica:on & resolu:on determine what can be seen by the microcope •  Magnifica:on = Expanding something in apparent size •  Resolu:on = Defined as the closest spacing of two points which can be dis:nguished as separate en::es. •  At higher magnifica:on, a microscope with greater resolu:on is required. Use Light microscopy to see live cells, colour and whole :ssues. Sec:oned plant stem with wood Resolu:on limit = 2 µm Nucleus labeled in Blue and Mitochondria labeled in Green (Fig 1 ­...
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