This hydrogel scaffold was then modified with an rgd

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Unformatted text preview: α -hydroxy acid) diacrylate. This hydrogel scaffold was then modified with an RGD peptide sequence. In this experiment, the RGD sequence was synthesized in one of two forms: a) RGD coupled to a PEG spacer (MW = 3,400 Da) and attachment site moiety (***), or b) RGD coupled to the attachment site moiety (***) with no spacer as shown below. (The polymer used to fabricate the hydrogels has a MW = 8,000 Da.) a) b) 1) What is the RGD sequence? (Sketch the sequence of amino acids showing all atoms and indicating peptide bonds). (5 pts) 2) Why did the researchers attach the peptide sequence RGD to their hydrogel? What is the role of RGD in cell – matrix interactions? (6 pts) 3) Which form of the RGD sequence peptide will yield the highest cell attachment per given number of peptides when attached to the polymer scaffold? (2 pts) Justify your answer. (2 pts) Hint: Look at the slides of Lecture 20 comparing Carmeda and Trillium approaches Medtronics uses for bioactive surfaces....
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