Unformatted text preview: α -hydroxy acid)
diacrylate. This hydrogel scaffold was then modified with an RGD peptide
In this experiment, the RGD sequence was synthesized in one of two
forms: a) RGD coupled to a PEG spacer (MW = 3,400 Da) and
attachment site moiety (***), or b) RGD coupled to the attachment site
moiety (***) with no spacer as shown below. (The polymer used to
fabricate the hydrogels has a MW = 8,000 Da.) a) b) 1) What is the RGD sequence? (Sketch the sequence of amino acids
showing all atoms and indicating peptide bonds). (5 pts)
2) Why did the researchers attach the peptide sequence RGD to their
hydrogel? What is the role of RGD in cell – matrix interactions?
3) Which form of the RGD sequence peptide will yield the highest cell
attachment per given number of peptides when attached to the
polymer scaffold? (2 pts) Justify your answer. (2 pts)
Hint: Look at the slides of Lecture 20 comparing Carmeda and
Trillium approaches Medtronics uses for bioactive surfaces....
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This note was uploaded on 01/30/2014 for the course ENGRI 1190 taught by Professor Grubb during the Fall '07 term at Cornell.
- Fall '07