Unformatted text preview: shape of double helix ‐bases provide specifics, ex restriction enzyme binds to specific nucleotide sequence ‐2/3 happen but UNSPECIFIC Why do denatured cells typically not refold? 1. salt concentration different 2. other folded proteins prevent folding 3. plasma membranes 4. other partially unfolded proteins prevent folding ‐in cell, very crowded ∴ interact with hydrophobic stretches in unfolded and hydrophilic in folded Why do you die when drinking a 1L solution containing 200g NaCl, but not one containing 200g of glucose? 1. glucose tastes better 2. glucose molecule weighs more 3. glucose is less toxic 4. glucose is less osmotically active 5. none of the above ‐NaCl is much smaller ∴200g in 1L = many more molecules (molarity matters!) ‐if same amt of molecules, same osmotic effect Why are sugars added to lipids in the Golgi found on the outside of the cell membrane? 1. Golgi enzymes are in the Golgi lumen (interior) 2. Golgi enzymes are on the outside of the Golgi 3. inner leaflet of Golgi will be the outer leaflet of the cell membrane 4. inner leaflet of Golgi will remain out of the cell membrane ‐note: viruses manipulate this mechanism in order to enter the cell Order mitochondria (1), nucleus (2), golgi (3), cell membrane (4) in terms of relatedness to endomembrane system? (most to least) 1. 2, 3, 4, 1 ‐nucleus & golgi are part of endomembrane system 2. 4, 2, 3, 1 ‐constant exchange of vesicles makes cell membrane more related than mitochondria 3. 2, 3, 1, 4 4. 3, 2, 4, 1 5. 3, 1, 2, 4...
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- Spring '13