Another approach might be to mutagenize the atp

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Unformatted text preview: reactive groups. F. The products of the reaction. Q 6 (this is a challenge question) An assay to detect altered superhelical torsion in DNA has been recently developed and used to explore the effect of the chromatin remodeling protein SWI/SNF on DNA topology. The assay uses cruciform formation as a means to measure local superhelical tension in DNA. The DNA sequence shown below contains inverted repeats that form a cruciform structure in negatively supercoiled DNA (see part B in the figure shown below). Cruciform structure can be detected by electrophoresis after cleavage of the DNA by Endo VII. Endo VII introduces bilateral cleavages close to the cruciform/B-DNA junction, generating a double-strand break. A restriction enzyme Ava I is also used in the assay to linearize the DNA. The site of cleavage by the AvaI restriction enzyme is shown in Part A in the figure. Three different versions of the DNA substrate shown in part A are used. In lanes 1- 4, the substrate is supercoiled. In lanes 5- 8...
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