MCB121+W14+practice+problem+set+1.2

B this enzyme uses which of the following to initate

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Unformatted text preview: e culture line and perform the following analysis. You synchronize the cells by arresting the cells in G1 of the cell cycle (prior to DNA replication) followed by release from arrest. Thirty minutes after the release from the arrested condition you incubate cells with high levels of 3H- labeled thymidine. After 15 minutes of labeling you change the medium to reduce the concentration of labeled thymidine and incubate an additional 15 minutes. You visualize the incorporated label on an autoradiograph of the replicating DNA and see the following patterns. A. On the diagram above, mark the likely site of all origins (O) and their corresponding replication forks (RF). Make sure it is clear which forks are part of the same replicon. B. Indicate...
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This note was uploaded on 02/03/2014 for the course MCB MCB 121 taught by Professor Gasser,burgess during the Winter '11 term at UC Davis.

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