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Unformatted text preview: , the substrate has been linearized prior to the assay using AvaI. In lanes 9- 12, the substrate is a relaxed circular DNA. Note that relaxed circular DNA migrates slower on the gel than the linear form. Please answer the following questions based on the data shown in Part C: A. (5 points) Why did the DNA in lane 2 migrate faster in the gel than the DNA in lane 9? Gel electrophoresis resolves DNA molecules based on both shape and size. Circular DNA molecules migrate in a gel at a rate proportional to their compactness. Supercoiled DNA is more compact than a relaxed circle. The DNA in lane 2 is supercoiled while the DNA in lane 9 is relaxed. Therefore the differences in mobilities is likely due to differences in the levels of supercoil...
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This note was uploaded on 02/03/2014 for the course MCB MCB 121 taught by Professor Gasser,burgess during the Winter '11 term at UC Davis.

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