MCB121+W14+practice+problem+set+1.2

The dna sequence shown below contains inverted

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Unformatted text preview: NA using zig- zag lines. C. Indicate all relevant 5’ and 3’ ends D. Indicate direction of fork movement using arrows. E. Mark the region where the origin of replication would be Q 5. Draw a diagram illustrating the addition of a dNTP to an RNA primer during DNA synthesis. You do not have to draw the structure of the bases or of the template DNA. Be sure to include the following: A. The last rNMP of the primer and the incoming dNTP. B. The chemistry of the reaction indicating the relevant reactive groups. C. The products of the reaction. Q 6 (this is a challenge question) An assay to detect altered superhelical torsion in DNA has been recently developed and used to explore the effect of the chromatin remodeling protein SWI/SNF on DNA topology. The assay uses cruciform formation as a means to measure local sup...
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