MCB121+W14+practice+problem+set+1.2

This protein can also interact with dna polymerase

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Unformatted text preview: ecifically to the protein (this is typically done by injecting purified protein into rabbits and purifying the raised antibodies from its blood serum several weeks later). In method 1, cells fixed to a microscope slide can be treated with the antibody followed by addition of a secondary antibody that is conjugated to a fluorophore and binds to the Ig region of the first antibody. The protein of interest can microscopically visualized indirectly by exposing cells to UV light. For the second method you could use chromatin immunoprecipitation (ChIP). These steps include 1) crosslinking DNA and its bound protein, 2) lysing cells and shearing DNA to small (~500 bp fragments), 3) adding antibody of protein of interest followed by precipitation of the protein- DNA- a...
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