A
C, T, N
T, N
T, N
T, N
B
C, T, N
C, T, N
C, T, N
C
C, T, N
C, T, N
D
T, N
E
Proline
Hydrogen
Shift (ppm)
Integration
Multiplicity
A
4.25
1
quad of d
B
2.00
1
obscured by amine
C
2.21
1
multiplet
D
1.90
1
obscured by amine
E
1.82
1
multiplet
F
3.55
1
multiplet
G
3.41
1
multiplet
COSY, NOESY, TOCSY
A
B
C
D
E
F
A
B
C, T, N
C
C, T, N
C, T, N
D
T, N
C(messy),T
C, T, N
E
T, N
C, T
C, T, N
C(messy), T
F
T, N
T, N
T, N
C, T, N
C, T, N
G
T, N
T, N
T, N
C, T, N
C, T, N
C, T, N
To start, we looked at the aromatic region of the 1D NMR.
There we saw two
peaks, one at 7.31 ppm and one at 8.6 ppm.
Looking at the shift list for amino acids,
these peaks corresponded to peaks for His (8.12 ppm and 7.14 ppm expected).
We then
looked at the integration values for that peak and found that the two peaks corresponded
to a hydrogen in the alpha region at 3.95 ppm.
We then looked to the COSY and found