Week 3 Review Sheet answers

Week 3 Review Sheet answers - Final Review Sheet Week 3...

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Final Review Sheet – Week 3 Lecture 7 - 1/23/08 Main Points: Enzyme kinetics: Key terms are Vmax and Km. You need to know the basis for each, the units, why Km is used as a reflection of the affinity of the enzyme for its substrate. We discussed how to plot the data on both Michaelis-Menten and Lineweaver- Burk plots, and the relative advantage of the latter for determining Km and Vmax. Reversible inhibitors can tell a lot about the enzyme. You need to be able to represent effect of these inhibitors with both types of graphs. Sample Questions 1. Define Vmax and Km. Vmax is the maximum rate at which a given number of enzyme molecules can work, a function of the turnover number. Km is the concentrations of substrate at which the reaction is running at 1/2 Vmax. 2. If two enzymes use the same substrate and one has a higher Km than the other, which will be active when the substrate is limiting? Explain. The Km is really a reflection of the affinity of the enzyme for its substrate.
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This note was uploaded on 04/08/2008 for the course BIOL 301 taught by Professor Tepperman during the Winter '08 term at University of Cincinnati.

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Week 3 Review Sheet answers - Final Review Sheet Week 3...

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