Lecture 7 Enzyme Kinetics

Lecture 7 Enzyme Kinetics - Lecture 7 Enzyme kinetics Basic...

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Lecture 7 Enzyme kinetics Basic set up of experiment Michaelis-Menten plot • V max and K M Lineweaver-Burk Reversible inhibitors Inhibitors in medicine Thermodynamics Energy/work/relationship to biology
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Points for the Day Enzyme kinetics can provide information about the efficiency of enzyme-substrate interaction Understanding what inhibits enzymes can be useful in developing new drugs Reactions in biological systems must follow the basic rules of thermodynamics
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Enzyme Kinetics Measure rates of reaction Used to study fundamental properties of enzyme (K M ) Study of inhibitors provides information about active site of enzyme Inhibitors developed may be used medically 0
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Measure the rate of product formation at specific amount of enzyme, specific amount of substrate For this combination of enzyme and substrate, you will get a “rate” = product formed/time μ M 0 PAY ATTENTION TO UNITS!!!!
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Do the same experiment, but change the amount of substrate (keeping enzyme constant) 0
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This note was uploaded on 04/08/2008 for the course BIOL 301 taught by Professor Tepperman during the Winter '08 term at University of Cincinnati.

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Lecture 7 Enzyme Kinetics - Lecture 7 Enzyme kinetics Basic...

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