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Lecture 12 Membranes II then transport

Lecture 12 Membranes II then transport - Lecture 12...

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Lecture 12 Membrane proteins More methods of study of membrane proteins Membrane fluidity Lipid rafts Protein mobility in the membrane Membrane transport General mechanisms Energetics of solute movement Diffusion
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Main Points of the Day Certain areas of the membrane have altered fluidity Proteins may or may not be mobile in the membrane How small molecules cross the membrane is a function of their structure
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Methods of Study of Integral Proteins Freeze Fracture Using detergents Membrane sidedness Hydropathy plots – analysis of transmembrane domains 0
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Determining Sidedness Erythrocyte Some proteins exposed only on outside, some only on inside, some both 0
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Experiment Use enzymatic reaction to add 125 I to proteins exposed on surface. Only externally exposed proteins are labeled. Make ghosts, Iodinate these. Now both externally and internally exposed proteins internally labeled 0
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0
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Results Whole cell Ghosts 2 3 2 3 4 5 1
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