Lecture 12 Membranes II then transport

Lecture 12 Membranes II then transport - Lecture 12...

Info iconThis preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 12 Membrane proteins More methods of study of membrane proteins Membrane fluidity Lipid rafts Protein mobility in the membrane Membrane transport General mechanisms Energetics of solute movement Diffusion
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Main Points of the Day Certain areas of the membrane have altered fluidity Proteins may or may not be mobile in the membrane How small molecules cross the membrane is a function of their structure
Background image of page 2
Methods of Study of Integral Proteins Freeze Fracture Using detergents Membrane sidedness Hydropathy plots – analysis of transmembrane domains 0
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Determining Sidedness Erythrocyte Some proteins exposed only on outside, some only on inside, some both 0
Background image of page 4
Experiment Use enzymatic reaction to add 125 I to proteins exposed on surface. Only externally exposed proteins are labeled. Make ghosts, Iodinate these. Now both externally and internally exposed proteins internally labeled 0
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
0
Background image of page 6
Whole cell Ghosts 2 3 2 3 4 5 1 Autoradiography of gels
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/08/2008 for the course BIOL 301 taught by Professor Tepperman during the Winter '08 term at University of Cincinnati.

Page1 / 29

Lecture 12 Membranes II then transport - Lecture 12...

This preview shows document pages 1 - 8. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online