Lecture 21 Internal Membrane Systems

Lecture 21 Internal Membrane Systems - Lecture 21 Methods...

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Lecture 21 Methods of study EM Autoradiography Fluorescence Differential centrifugation Use of mutations Rough ER Synthesis of proteins by at RER Synthesis of membrane lipids Post translational modifications Movement to Golgi via coated vesicles
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Main Points Proteins include targeting stretches of amino acids within their primary structure that can direct where the bulk of their synthesis occurs or where they end up Proteins made on bound ribosomes transit via vesicles Vesicle transit requires specific coating proteins for formation
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Pulse. Grains (in red) are over RER 17’ chase. Grains are in Golgi 120’ chase. Grains are in secretory vesicles Pancreatic Acinar Cells 0
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Movement of Protein Through a Living Cell
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Differential Centrifugation “Microsomes” are vesicles that form from ER when cells are homogenized Both rough and smooth microsomes can be isolated Finer separations through discontinuous gradients 0
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Study Secretion
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This note was uploaded on 04/08/2008 for the course BIOL 301 taught by Professor Tepperman during the Winter '08 term at University of Cincinnati.

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Lecture 21 Internal Membrane Systems - Lecture 21 Methods...

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