Carleton-Lecture-Animal models stroke and VaD

2008 the two common carojd arteries are temporary

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Unformatted text preview: blood flow to the enJre brain •  Produces wide spread injury to vulnerable cell populaJon in the brain including the neocortex, striatum and hippocampus •  Typically achieved via the occlusion of blood vessel(s) supplying the brain, applicaJon of a neck tourniquet or decapitaJon •  The two (2VO) and four vessel occlusion (4VO) models are most commonly used Global ischemic model: 2VO Yonekura et al. 2008 •  The two common caroJd arteries are temporary occluded •  Produces near complete ischemia in rodent species such as the gerbil that lacks posterior communicaJng arteries •  Five minute occlusion Jme is sufficiently produces death of CA1 neurons •  Severity of injury depends on occlusion Jme •  VariaJon in this model exists e.g it can be coupled with hypotension Global Ischemic model: 4VO •  Similar to the 2VO model but a liQle more surgically challenging. •  Performed in two stages, usually over two days •  Day 1: ligature is loosely placed around both common caroJd arteries (CCA) and the two vertebral arteries are electro- cauterized •  Day 2: both CCA are transiently occluded for a set period of Jme •  Relably reduces blood flow to <3% of control values in the hippocampus, neocortex and striatum •  6 to 10 minutes occlusion Jme produces almost complete death of CA1 neurons by day 3 post- ischemia Changes in blood supplies to the cortex and brain stem resulGng from clos...
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This document was uploaded on 02/03/2014.

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