Carleton-Lecture-Animal models stroke and VaD

Majority of stroke are thromboembolic in nature ie

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Unformatted text preview: lusion Jmes MagneJc resonance imaging of damage in ischemic stroke. Before 14 days 21 days Aher 30 mins MCAO in mice Zille M et al, JCBFM (2012) 32, 213- 231 Histological EvaluaJon of brain damage DemarcaJon of infarcted Jssue by Hematoxylin and eosin (H&E) staining •  Hematoxylin stain cell nucleus (blue) and eosin stains cytoplasm (pink) •  Allows visualizaJon of cell morphology •  Dead/dying cells appear pyknoJc (irreversible condensaJon of chromaJn MCAO in the rat Triphenyl tetrazolium chloride (TTC) staining •  Used to idenJfy metabolically acJve and inacJve Jssue •  TTC stains viable Jssue red as it is reduced to TPF (1,3,5- triphenlyformazan) and remains white in damage Jssue Zille M et al, JCBFM (2012) 32, 213- 231 Focal ischemic model: Endothelin- 1 injecJon •  Endothelin- 1 is a potent vasoconstrictor •  Works by binding receptors that are present on the vascular endothelium •  Reduces local blood flow causing focal lesion •  Can be applied topically anywhere on the cortex or injected into striatum to produce infarcJon Allard et al, Biomaterials, 2009 vol 30 (2) Focal ischemic model: Thromboembolic •  Commonly produced by injecJng thrombi or non- clot material (microspheres) into the extracranial arteries •  Cause occlusion of distal intracranial vessels •  Produces mulJfocal brain lesions •  Considered to more closely...
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This document was uploaded on 02/03/2014.

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