{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

The contol included only adipic acid and pimeric acid

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: troduction and Background 10 Purpose Chromatoplots is an in-development R package for the analysis of metabolomic data collected via gas chromatography mass spectrometry (GC-MS). The package is co-authored by Tengfei Yin and Michael Lawrence. This analysis attempts to validate the component-grouping step of the chromatoplots pipeline, by using exploratory data analysis (EDA) and clustering methods to identify the number of unique metabolites present in the total list of components. Since the raw data includes both replicates and samples that share a subset of common entities, we anticipate identifying one group for each unique metabolite, in which the number of leaves in that cluster is equal to the number of samples in which that metabolite is present. Eg metabolomics 1.2 Experimental Design This data was collected by Suh-yeon Choi using gas chromatography mass spectrometry (GC-MS) in the summer of 2009 to mimic a typical metabolomics experiment. It contains two samples which mimic two experimental units, such as wild type and treatment group, and a control. Each sample was prepared twice (experimental replicates) and two aliquots from each replicate was tested (technical replicates), for a total of 4 replicates for each sample. The control was not replicated. Samples contain an mixture of artificial metabolites, representing extracts from living organisms. Included are multiple metabolite classes (amino acids, sugars, sugar alcohols, and various organic acids) with wide range of retention times. Two unique metabolite mixtures (mix1 and mix2) represent different experimental conditions (e.g. control and treatment). Each mixture was created independently twice, mimicking biological replicates of each experimental condition Four metabolite mixtures (2 conditions X 2 replicates) were derivatized and analyzed using gas chromatography coupled to mass spectrometry (GC/MS) in two independent experiments as technical replicates, demonstrated in table 2. The chemicals, their concentration and presence...
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern