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Unformatted text preview: xoneme:
1. Remove membrane w ith non ionic detergent
2. S ubject axoneme to mild prteolytic treatment to r emove Nexin links
3. Added ATP
Cytoplasmic dynein dr ives minus end directed movement of organelles
Examples of cytoplasmic dyne in dr iven or ganelle movements: RER --> Golgi; Inw ar d movement of lysosomes
Also, bidirectional organelle movements on a single MT
Domain str ucture of kinesin: N-terminal motor/head, neck, tail/stalk and C-ter minal light chains
(coiled coil; head, etc)
Many kinesins ar e processiv e motors;taking many steps along the MT before defusing aw ay from
the MT (a single kinesin molecule/organelle may be sufficient fo...
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- Fall '13