Unformatted text preview: f. at high shear str ess, viscosity of cytoplasm is low
and exhibits little resistance to bead movement
Experiment al analy sis of diffusion barriers/ crowdedness of cy t oplasm:
1. Prepar e uniform-sized pr eparations of fluor escent dextran particles of different sizes from 5100nm in diameter.
2. Microinject into cultured cell.
3. Measure diffusion coefficient
--> dextran par ticles with diameter <50nm exhibited fr ee diffusion
--> >50nm exhibited significantly slower diffusion rates than in...
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- Fall '13
- Physiology, actin binding proteins, nm diameter, shear str ess, Unipolar actin bundles