BIO 102 Lecture 9 Notes

4 warm cells to 37 degrees and quantify at various

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: 4. Warm cells to 37 degrees and quantify at various times after w arming surface bound [pulse chase] LDL has protein component, lipid component [Why is there a gr adual increase in TCA soluble 125I and a gradual decrease in T CA insoluble (hep r esistant 125I)?] Digestion of TCA insoluble --> TCA soluble [What exper iment would demonstrate that the residual LDL surface binding at 30-120 minutes is specific or non-specific?] --you can put in non-labeled LDL and it stays the same, it means that it's nonspecific --if you put in non-labeled LDL and it all becomes 0, that means it's specific Made ferr itin labeled LDL (little black dots) -- demonstrates that LDL is internalized Classes of LDL Receptr Mutations leading to familial cholester olemia: 1....
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern