BIO 102 Lecture 3 Notes

Us e v ac uum ev aporat or f or c oating s pec imens

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Unformatted text preview: ponding s ide) 3. Us e v ac uum ev aporat or f or c oating s pec imens wit h met al (plat inum s pray ed down ont o s urf ac e of f reez e f rac tured membrane) In freez e f rac t ure et c hing, ic e is s ublimed away (from s olid --> gas ) aft er f rac ture t o rev eal inner membrane s urfac es and c y toplas mic s t ruc t ures Ex perimental analy s is as s es s ment of membrane protein lat eral mobilit y : -redis tribut ion of c ell s urf ac e antigens af t er mous e-human c ell f us ion -lec t in/antibody induc ed patc hing and c apping of c ell s urf ac e prot eins -FRAP/ / FLIP analy s is of f luores c ent ly t agged membrane prot eins Fry e and Edidin, 1970: The firs t demons tration of lat eral mobilit y of membrane prot eins wanted t o f ind out what would happen if y ou fus ed mous e/human c ell 1. Mak e antibodies t o c ell s urf ac e proteins of mous e c ell line in rabbit. Mak e ant ibodies to c ell s urf ac e proteins of human c ell line in mous e. 2. At low t emp, fus e mous e/ human c ells wit h Sendai v irus to generate mous e/hum...
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