BIO 102 Lecture 3 Notes

Ed on one s ide of t he helix func tional s ignif ic

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Unformatted text preview: dues orient ed on one s ide of t he helix . Func tional s ignif ic anc e: polar s ide groups interac t (lowes t energy s tat e) and c reat es a hy drophilic c hannel t hrough whic h hy drophilic molec ules c an go in/ out through Hy drophobic it y plot - learn what t his is A n a t o m y o f a n i n t e g r a l m e m b r a n e p r o t e i n : an ex t rac ellular/ luminal domain c an be gly c os y lat ed and s t abiliz ed by S-S bonds (there's a lot of s ugar in prot eins ) Ex t rac ellular s urf ac e of many c ells has a t hic k " s ugar c oating" In the pic ture: ly mphoc y t e has been s tained with a c arbohy drate s t ain prior t o f ix at ion and embedment Pot ent ial f unc tions : mec hanic al prot ec t ion, s erv e as ligands for rec eptors F r e e z e F r a c t u r e T E M : Experiment al det erminat ion of membrane prot ein t opography / organiz at ion wit hin the bilayer plane 1. Freez e c ells /t is s ues (v ery quic k ly ) in liquid N2 c hilled s olv ent 2. Cleav e the f roz en s pec imen wit h c ooled mic rot ome blade (s plit right down t he bilay er) Alway s f rac tures s imilarly : ex t rac ellular f ac e; p fac e (proteins s tay wit h c orres...
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