BIO 102 Discussion Paper 2 (Frye and Edidin) Notes

antibodies from the same species but a different

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Unformatted text preview: odies from the same species, but a different hisocompatibility complex (like self, but not exactly like self) -Making human antibody (injected human cells into rabbits with VA-2 cells) [because it w as mouse-mouse and human-rabbit, there ar e a lot mor e sites for antibody binding (fluorescence) in the human-rabbit so it'll fluor esce more brightly) How did t hey t est t heir primary ant ibodies? What are they testing? -specificity (vs. cross-r eactivity) -Why does injecting whole cells increase the likelihood of cross-reactivity? A lot more antibodies -Mouse and human antibodies tested in similar w ays -add mouse antibody to only human cells (if this w or ked, it would be non-specific and cross r eactive) -add mouse antibody to human cells + sendai virus and look for r eaction (w ant to make sur e sendai virus isn't affecting the membr ane) (if you flip mouse and human, it's just how they tested human antibodies) T he FC domain becomes the new antigen binding site for the secondary antibody primar y antibody: r abbit antibody against antigen A secondary antibody...
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