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Unformatted text preview: odies from the same species, but a different hisocompatibility complex (like self, but not
exactly like self)
-Making human antibody (injected human cells into rabbits with VA-2 cells)
[because it w as mouse-mouse and human-rabbit, there ar e a lot mor e sites for antibody binding
(fluorescence) in the human-rabbit so it'll fluor esce more brightly) How did t hey t est t heir primary ant ibodies?
What are they testing?
-specificity (vs. cross-r eactivity)
-Why does injecting whole cells increase the likelihood of cross-reactivity? A lot more antibodies
-Mouse and human antibodies tested in similar w ays
-add mouse antibody to only human cells (if this w or ked, it would be non-specific and cross
-add mouse antibody to human cells + sendai virus and look for r eaction (w ant to make sur e
sendai virus isn't affecting the membr ane)
(if you flip mouse and human, it's just how they tested human antibodies) T he FC domain becomes the new antigen binding site for the secondary antibody
primar y antibody: r abbit antibody against antigen A
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- Fall '13