Unformatted text preview: : marker-coupled antibodies dir ected against rabbit antibodies (antibody against
the antibody) Why make stable hybrid lines?
-Positive contr ol
-Gr een = mouse; r ed = human What is "ring r eaction"?
-looks like a ring w hen you focus on cell equator
M1/2-H1/2 = heterokaryon
M1/2-H1 or M1-H1/2
M1-H1 = mosaics What other types of cells would they see?
Parental (unfused); homokaryons; br oken cells (selection w ould theoretically prevent this from
Did not fix cells prior to staining/imaging; which could be problematic for imaging
4 theor ies: (look on paper )
4) stop cell from pr oducing antigens
stop ATP preventing protein synthesis still produced many mosaics, inhibited AT P and didn't find any change
in mosaic for mation impact: lowering temp...
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This note was uploaded on 02/05/2014 for the course BIO 102 taught by Professor Chandhoke during the Fall '13 term at Yale.
- Fall '13