PROC FOR QUES 2 [S]

PROC FOR QUES 2 [S] - PROCEDURE FOR QUESTION 2 EFFECT OF...

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1 PROCEDURE FOR QUESTION 2: EFFECT OF SUBSTRATE CONCENTRATION 1. You will follow the procedure you used in Exercise 6 , the baseline experiment (See pages 10- 11) but you will use the new mixing table. You will need to do a new Baseline experiment using 2 ml of hydrogen peroxide (substrate) - Tubes 2 and 3. You will have to do another baseline because the turnip extract will be different from that made in the previous lab. The baseline run will be the positive control – which contains the variable for which you are testing . You will compare all other conditions studied to the baseline condition. Note that in this case Tube 1 is your “blank” as well as the “negative control”. You will be using Tube 1 to calibrate the spectrophotometer for each set of conditions (2 & 3), (4 & 5), (6 & 7), (8 & 9), (10 & 11), (12 & 13), (14 & 15) and (16 & 17). 2. As part of your protocol, you should refer to the Mixing Table for all conditions to write your predictions. Mixing Table: Effect of Substrate Concentration on the Activity of Turnip Peroxidase
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PROC FOR QUES 2 [S] - PROCEDURE FOR QUESTION 2 EFFECT OF...

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