Possible Experiments for the last week updated

Possible Experiments for the last week updated - type of...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Possible Experiments 1. We have other substrates of the TCA cycle available (citrate, α -ketoglutarate, and isocitrate). Some reactions might be assayed with the assay we used for succinate dehydrogenase, while the assay might be less likely to work with others. Develop a hypothesis based on your understanding of the assay as to which might be better substrates for the DCIP assay than others and test your hypothesis. 2. We have a compound, malonate (not to be confused with malate), which is supposed to be an inhibitor of succinate dehydrogenase. Devise an experiment to determine what
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: type of inhibitor it is (competitive or non-competitive). 3. Most inhibitors are specific for the enzyme they inhibit. Devise a test to determine whether it is true that malonate is specific for succinate dehydrogenase. 4. In the first week, we looked at broken preparations of mitochondria compared to whole mitochondria. Based on your understanding of this experiment, devise a hypothesis concerning how other enzymes in the TCA cycle might behave. Devise an experiment to test your hypothesis....
View Full Document

This lab report was uploaded on 04/08/2008 for the course BIOL 301 taught by Professor Tepperman during the Winter '08 term at University of Cincinnati.

Ask a homework question - tutors are online