Succinate Dehydrogenase Lab

Succinate Dehydrogenase Lab - Succinate Dehydrogenase in...

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Succinate Dehydrogenase in  Mitochondria Purpose:  to investigate activity of  succinate dehydrogenase in the  presence of increased enzyme,  increased substrate, and various  other conditions Broken mitochondria Also research malate DH No azide Heat
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Approach: Isolate mitochondria from cauliflower Measure the SDH activity using a spec and  DCIP, an artificial e -  acceptor Determine the normal rate of the reaction by  calculating the change in ABS Succinate Dehydrogenase in  Mitochondria
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Succinate Dehydrogenase Removes electrons from its substrate  succinate  and uses them to reduce  FADH to FADH 2 FADH 2  is normally oxidized by the  electron transport chain (ETC) Here, an artificial electron acceptor,  DCIP, is used to trap the electrons
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DCIP “Catches” e -  before ETC Sodium azide inhibits ETC Using sodium azide increases the chances 
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This lab report was uploaded on 04/08/2008 for the course BIOL 301 taught by Professor Tepperman during the Winter '08 term at University of Cincinnati.

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Succinate Dehydrogenase Lab - Succinate Dehydrogenase in...

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