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BS54-Exam2-2005 - NAME TF 2005 Biological Sciences 54 EXAM...

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NAME: ___________________________________ 2005 TF: ________________________________________ Biological Sciences 54 EXAM 2 A. Be sure to write your name on the top of each of page of the examination. B. Write each answer only on the same page as the pertinent question. THE SPACE PROVIDED IS MEANT TO BE SUFFICIENT BE CAREFUL & BE CONCISE. If absolutely necessary, use the back of that page to continue the answer. C. IF YOUR ANSWER CANNOT BE READ IT CANNOT BE GRADED. D. Remember, if you do not show your reasoning, you cannot get partial credit. E. The point value for each question is noted. ALLOCATE YOUR TIME WISELY. F. Read carefully, think before you write, good luck, and do well! page 2: __________ (25 pts) page 3: __________ (25 pts) page 4: __________ (25 pts) page 5: __________ (25 pts) TOTAL: _______ (100 pts)
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NAME:___________________________________ Page 2 of 5 1. (8 pts.) You have two confluent plates of epithelial cells growing in medium - Plate X is untreated while Plate Y is treated with EGTA, a Ca 2+ chelator. When both plates of cells are wounded by dragging a needle twenty times across each plate, you observe that after 6 hours the number of surviving cells in Plate X is higher than in Plate Y. Explain the difference in survival highlighting what you know about the Patch Repair hypothesis. 2. (17 pts.) You are interested in the process of mitochondrial movement in neuronal cells. The graph below indicates the results of experiments you carried out in cells using the drugs colchicine (disrupts microtubules) and cytochalasin D (disrupts actin microfilaments). The graph shows the effects of these drugs on the relative anterograde mobility of mitochondria towards the axon terminus. Values are relative to the movement observed in the absence of either drug (control), which has been arbitrarily set to 100%.
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