BS54-PS5-2006-KEY

BS54-PS5-2006-KEY - Problem Set 5 answer key 1 1 pt Order...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Problem Set 5 answer key 1. 1 pt. Order: C-A-B 4 pts Justifications: Mutant A- calcium release cannot be activated even in the presence of non-hydrolyzable IP3, so the problem is probably in the calcium channel itself. Mutant B- Calcium release is triggered in both experiments, but gene expression is not observed. This indicates something wrong downstream of calcium release. Mutant C- no effect is observed unless IP3 is added. Probably something wrong with the receptor or PLC, such that PIP2 is never cleaved to produce IP3 and DAG. Functions: C could be the receptor or PLCg, A is probably the Calcium channel, and B is PKC or any transcription factor targets. 2. A) 3 pts. Protein X is a substrate of APC Accordingly, at the onset of anaphase, it becomes polyubiquitinated, producing higher molecular weight versions of Protein X. The polyubiquitination targets Protein X for degradation by the proteosome. Thus, in telophase, no Protein X remains to be detected by western blot.
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

Ask a homework question - tutors are online