Lab 3

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LABORATORY #3 Measuring the production of oxygen in an aqueous environment of the specie Cabomba, With temperature as a variable. Janneke Zijsling 212225421 Lab 18- Section B T.A: Sanjana
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INTRODUCTION Photosynthesis is a sensitive process that responds in different ways depending on the environment and its changes. (Vargas and Cordero 2012). Recent studies have shown that there are many factors that affect the photosynthetic rate of plants. One study showed that the photosynthetic rate of tropical forest trees in the Northern Lowlands of Costa Rica is affected when the temperature increases. (Vargas and Cordero 2012). Using studies like the one mention above as a reference helped this experiment to formulate a hypothesis: The increase of temperature by 10C above room temperature (22C) will increase the photosynthetic rate, compare to the value at room temperature of the specie Camboba.
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MATERIALS AND METHODS The materials used in the experiment three groups of materials. First the Manometric system which included three stoppers, 3 syringes, three pipettes, one tank and a source of light. Second, the materials that were used to make the Manometric system work: Three glass tubes, the plant material, which in this case was the specie cabomba, the conditioned or pond water, one plastic Pasteur pipette and the indicator (blue water). Finally additional materials like the test tube rack which was used to hold the glass tubes. The way the experiment was performed included different steps: The first step in this experiment was to weigh out 3 to 4g of the plant that is going to be studied. Was important to zero the balance before weighing the plant. Two samples of the plants were needed, one for each experimental tube. After the plant was weighted, it was placed into the experimental tubes. Each tube was filled with pond water. The experiment needed to have a control sample to compare with the experimental tubes. The third tube was filled with pond water without adding the organism. The next step was to set up the Manometric system. Each tube stopped with a stopper. On one side of the stopper the syringe was introduced, and in the other side the pipette. Before plugging in the pipette, a small drop (3mm to 5mm) of blue water was added to the
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