QUES 1 - [E] - PROCEDURE FOR QUESTION 1 EFFECT OF ENZYME...

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1 PROCEDURE FOR QUESTION 1: EFFECT OF ENZYME CONCENTRATION 1. You will follow the procedure you used in Exercise 6 , the baseline experiment (See pages10-11) but you will use the new mixing table. You will need to do a new Baseline experiment using 1 ml of extract (enzyme) - Tubes 2 and 3. You will have to do another baseline because the turnip extract will be different from that made in the previous lab. The baseline run will be the positive control which contains the variable for which you are testing. You will compare all other conditions studied to the baseline condition. Note that in this case Tube 1 is your “blank” as well as the “negative control”. You will be using Tube 1 to calibrate your Spec 20. You will use Tube 1 to recalibrate the spectrophotometer for each set of conditions (2 and 3), (4 and 5), (6 and 7), (8 and 9), (10 and 11), and (12 and 13). 2. As part of your protocol, you should refer to Mixing Tables for all conditions to write your predictions. Mixing Table: Effect of Enzyme Concentration on the Activity of Turnip Peroxidase
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This note was uploaded on 04/07/2008 for the course BIOSCI 0050 taught by Professor Carollafarve during the Fall '07 term at Pittsburgh.

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QUES 1 - [E] - PROCEDURE FOR QUESTION 1 EFFECT OF ENZYME...

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