Bio110AntibioticResistanceLabManual2013_

Forthisprocedureyouwillbeworkinginagroupof6atyourbench

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Unformatted text preview: e agar surface. (Careful! They bounce!) Replace the lid on the Petri dish, put the capped test tube back in the test tube rack, and then gently slide the Petri dish side‐to‐side and in a circular motion on the bench top. This will spread the bacteria evenly over the entire agar surface, instead of leaving them concentrated in the middle of the dish. 7. Use the same set of beads for each of your 3 plates – when you are done with the 10‐6 plate, pour the ‐4 ‐2 beads gently onto the 10 plate, and finally the 10 plate. When you are done with all 3 plates, dispose of the beads into the red biohazard bag on your bench. 8. When finished, each person should invert and stack their three Petri dishes. Tape your stack of 3 dishes together and clearly label the tape with your name and section number. 9. Leave all plates at your bench. The prep crew will take the dishes to an incubator for ~24 hours and then to a cold room for storage until you are ready to count the bacteria present on the plates next week. Note: When you are finished, make sure all used pipette tips and gloves are disposed of properly in the waste containers provided and clean your work area with 70% ethanol before you leave the lab. If you have any questions concerning sterile technique and laboratory safety, please consult your TA. ASSIGNMENT: 1. Complete Homework Assignment #5 (provided by your TA) to demonstrate your understanding of serial dilution calculations. See syllabus for due date. 6 Week 2 Experiment Part A (Continued) Observations Collect your Petri dishes with the bacteria that you plated during the last lab. Remove the tape from the stack and examine your plates for bacterial colonies. Record which plates contain individual bacterial colonies and will be countable. Experiment Part B: Using PCR to Amplify Specific Genes for Kanamycin Resistance. Create a heading in your notebook: “Experiment Part B: Using PCR to Amplify Specific Genes for Kanamycin Resistance” Procedure – Experiment Part B Record any intentional or unintentional modifications to this procedure in your science notebooks 1. For this procedure, you will be wor...
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This note was uploaded on 02/06/2014 for the course BIO 110 taught by Professor Hass during the Fall '11 term at Pennsylvania State University, University Park.

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